Development of a concentration‐controlled sequential nanoprecipitation for making lipid nanoparticles with high drug loading

Lipid-based nanostructures have garnered considerable interests over the last two decades, and achieved tremendous clinical success including first approval of a liposome (Doxil) for cancer therapy in 1995 recent COVID-19 mRNA lipid nanoparticle vaccines. Compared to liposomes which bilayer surrounding an aqueous core, nanoparticles with particle structure several attractive advantages encapsulating poorly water-soluble drugs such as better stability due structure, high drug encapsulation efficiency because pre- or co-drug-loading strategy. While many studies reported synthesis hydrophobic encapsulation, precise control loading remains significant challenge. This work reports new concentration-controlled nanoprecipitation platform technology fabricating tunable up 70 wt%. method is applicable wide range from very slightly hydrophilic. Using this facile method, exhibited excellent properties small size, narrow size distribution, good stability, showing great promise future delivery applications.